 Solution

Set $q=y-x$ and $z_t=x+tq$. The definition of pushforward measure gives the confinement energy $\mathcal{V}[{\mu }_t]=\int V(z_t)d\Pi$. The chain rule and differentiation under the integral sign, whenever justified, giveFor example, bounded Hessian matrix is sufficient: it gives $|\nabla V(z)|\le C(1+|z|)$, and the first two differentiated integrands are bounded by $C^{\prime }(1+|x{|}^2+|y{|}^2)$. The dominated convergence theorem applies because $\Pi$ has finite second absolute moments. Compactly supported endpoints also suffice because all interpolating points lie in one compact set.

The printed growth assumption alone does not ensure a classical second derivative. Here is a counterexample within the stated assumptions. On the line take the bounded nonnegative smooth functionand the probability measures $\mu ={\sum }_n{p}_n{\delta }_{n-1/2}$, $\nu ={\sum }_n{p}_n{\delta }_{n+1/2}$. Both have finite second absolute moments. Their translation transport plan is optimal: its cost is one, while every coupling has cost at least the square of its mean displacement, also one. At $t_0=1/2$, $z_{t_0}=n$, so $F(t)=\mathcal{V}[{\mu }_t]$ satisfies $F(t_0)=0$. Each summand is nonnegative, and the Fatou lemma givesThus the requested classical identity requires an additional integrability assumption on the Hessian matrix; quadratic growth controls the energy but not its second derivative.

For a confinement potential $V$ that is a $\lambda$-strongly convex function, the derivative formula, where valid, yieldsThe underlying displacement convexity conclusion is valid under the printed assumptions without that extra differentiation hypothesis. Apply convexity of $V(x)-\lambda |x{|}^2/2$ pointwise and integrate:The same argument on every subinterval makes $t\mapsto \mathcal{V}[{\mu }_t]-\lambda D^2{t}^2/2$ convex, so the second derivative lower bound also holds in the sense of distributions.