Let the real spline have distinct knots, order , nonzero endpoint coefficients, and no identically zero knot cell in its support. It and its first derivatives vanish at both support endpoints. If is its number of interior distinct zeros, there are initially zero components. Each differentiation through order increases this count by at least one: a nonzero gap between zero components has an interior extremum, and the two endpoint zeros remain separate. The final derivative is continuous piecewise linear on cells, and each cell can meet at most one zero component unless it is identically zero, in which case all its zeros belong to the same component. It therefore has at most zero components. Thus , proving the bound.

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