Considerations when quantitating protein abundance by immunoblot by Alicia A. McDonough and others (2015, 10.1152/ajpcell.00400.2014)

By Alicia A. McDonough, Luciana C. Veiras, Jacqueline N. Minas, Donna Lee Ralph.

Publisher: American Physiological Society.

Published in American Journal of Physiology-Cell Physiology.

Online text: pmc.ncbi.nlm.nih.gov/articles/PMC4360027/

Cited passages: McDonough and others, “Consider the Loading Controls”, Figure 4: sample doubling changes claudin detection but not the actin signal; McDonough and others, “Consider the Specificity of the Antibody for the Target” and “Consider the Linearity of the Detection System”; McDonough and others, “Consider the Specificity of the Antibody for the Target”, Figure 2: positive tissue, knockout tissue and antibodies to different termini.