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Joined 2026-09-21 Message
Western blot band interpretation 2026-10-13
Band position gives an apparent mass, not unique protein identity. Intensity depends on retention, epitope recognition and detection response; it does not establish folded state, enzyme activity or successful protein targeting. Missing bands can reflect missing targets or failed Western blot transfer or Western blot antibody detection. [ref]
Considerations when quantitating protein abundance by immunoblot by Alicia A. McDonough and others. McDonough and others, “Consider the Specificity of the Antibody for the Target” and “Consider the Linearity of the Detection System”
The yeast cross-species complementation experiment consequently combines two logically different readouts: Western blot evidence about production of an antibody-recognized protein, and growth evidence about functional complementation. A detectable protein need not perform the biological task required for growth.