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Domenico Pacini tested the origin of atmospheric ionizing radiation by placing a sealed measuring instrument below the surface of water. His experiments compared the rate of discharge near the surface with that measured underwater.

A lower discharge rate underwater showed that part of the ionization came from radiation attenuated by the overlying water. Instrumental leakage and radiation from surrounding material also had to be considered. The result challenged explanations assigning all the penetrating radiation to local terrestrial sources, using shielding rather than altitude as the controlled comparison.^[ref]

Victor Franz Hess's 1912 series used two sealed, 3-mm-walled ionization chambers with Theodor Wulf's fiber electrometers. Karl Bergwitz suggested internal plating; Günther & Tegetmeyer improved focusing. Basket-mounted ground comparisons, insulation-loss measurements, simultaneous detectors and barograph-derived heights supplied checks. The highest flight reached 5350 m; its comparisons came from Vienna and after landing, without a launch-site baseline.^[ref]

A third, thin-walled chamber admitted ambient air. Hess multiplied its readings by $750/b$, with pressure $b$ in millimeters of mercury, warning that vessel-wall contributions might violate this scaling. He recorded outside temperature but applied no absolute-temperature correction. Night flights, an eclipse and weather observations constrained alternatives to radiation from above; the eclipse argument addressed directly propagating solar radiation.^[ref]

The electrometers read gradual electric potential loss caused by continuing air ionization. The low-altitude dip and strong high-altitude rise favored an extraterrestrial contribution, without measuring proton composition.^[ref] An ionization chamber integrates electric charge: one energetic particle may create many ion pairs, several particles may contribute to one reading, and a photon can create charged particles in the gas or walls. A voltage-loss reading is therefore neither a count of primary cosmic rays nor a chemical analysis of them.

Domenico Pacini supplied independent evidence: in June 1911 he compared a copper-box-enclosed electroscope at the sea surface and 3 m underwater, more than 300 m offshore above an 8-m-deep seabed. Reduced underwater ionization, stable electrical loss between runs, and a lake comparison supported an external contribution independent of soil radioactive decay. Water provided shielding; this established a different constraint from Hess's altitude comparison.^[ref]

By Domenico Pacini. Il Nuovo Cimento VI/3, 93–100 (1912). Translation and commentary: Alessandro De Angelis. Inspected arXiv v3 (2017), including original facsimile.