Sigma procedure SPBAPN02.001, A6012, revised 22 December 1997. Sigma is the institutional issuer; Merck Millipore hosts this copy. No personal byline or original publication date is established.
Cited passages: p. 1 Principle, Conditions and Reagents; p. 2 Procedure and Calculations; p. 3 Notes.
Additional cited passages: p. 2, approximately five-minute recording and linear slope; p. 3, notes 1–2, substrate quality and 40–60 percent inhibition criterion.

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