fluorescence recovery after photobleaching follows fluorescence return to a bleached region as unbleached labeled molecules redistribute. Recovery depends on geometry, transport, binding and immobile fractions; it is not a direct measurement of every aspect of membrane fluidity.
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Fluorescence recovery after photobleaching (FRAP) is a quantitative imaging technique used primarily in cell biology to study the dynamics of fluorescently labeled molecules within live cells. The method provides insights into the movement and interactions of proteins, lipids, and other biomolecules in cellular membranes and structures.