Gene prediction infers candidate genes and their boundaries from genomic sequence and supporting data. Models can recognize coding patterns and splice signals, while transcript and comparative evidence help constrain possible structures. Predictions can miss short genes, confuse duplicated regions or propose unsupported splice forms. Experimental transcript evidence can improve a gene model, but observation of a transcript does not alone establish that it produces a functional protein. [ref]

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Gene prediction refers to the process of identifying the locations of genes within a genome. This involves determining the sequences of DNA that correspond to functional genes, as well as predicting their structures, including coding regions (exons), non-coding regions (introns), regulatory sequences, and other features that are essential for gene function and expression.