Tracking descendants through an inherited genetic label or sequence change. Labelling specificity, timing and independent activation determine what ancestry can be inferred.
Failure to recover a lineage label from a sampled descendant. No recovered label is therefore not equivalent to proof of absent ancestry.
Using accumulated CRISPR-induced sequence changes as inherited records of ancestry. Nested scars support a lineage tree only after considering recurrent edits, deletion of earlier scars and missing observations.
A lineage-recording approach coupling inherited CRISPR barcode edits with single-cell transcriptomes. It can relate cell identity to developmental ancestry while retaining the limitations of incomplete barcode recovery and edit homoplasy.[ref]
An inherited sequence label used to distinguish cell ancestries. Independent acquisition of the same barcode, loss of the readout and nonuniform barcode frequencies can all confound a clonal inference.
Independent cells receive the same apparent label. If barcode probabilities are , two independent founders match with probability , rather than necessarily the inverse of the nominal library size.
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