H. Vink investigated the functional properties of the endothelial glycocalyx. In work with B. R. Duling, the researcher compared the domains occupied by different components of blood within mammalian capillaries.[ref]
The 1996 experiments combined bright-field observations of blood cells with fluorescence microscopy of circulating macromolecules in hamster muscle. The study also included a light-and-dye perturbation, testing the relationship between the observed surface layer and the space accessible to flowing components.[ref]
Identification of distinct luminal domains for macromolecules, erythrocytes, and leukocytes within mammalian capillaries by H. Vink and B. R. Duling. Methods and indexed primary abstract: microscopy modalities and light-dye treatment
An illuminating measurement question is “accessible to what?” A boundary inferred from a fluorescent tracer need not coincide with the vessel wall or with the path occupied by a blood cell. Comparing components makes the probe's physical interaction with the surface part of the interpretation, rather than assuming that every measured width denotes the same anatomical opening.
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