fluorescence microscopy forms images from spatially resolved fluorescence. Probe partitioning, optical resolution and photophysics constrain what a fluorescent pattern establishes about molecular composition or contact.
Live-cell imaging follows living biological cells over time. Labels, illumination and immobilization must be checked for effects on the process being measured; an apparently sharp image is not sufficient evidence that the cell remains physiologically unperturbed.
Imaging of biological events in a living organism. Repeated cell tracking can establish event ordering but requires controls for phototoxicity, imaging access and sampling.

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