I. Eronen is the first author of a 1985 study isolating and characterizing keratan sulfate from human brain. The researchers identified a sulfated carbohydrate fraction with repeating galactose and N-acetylglucosamine units. Its susceptibility to a keratan-sulfate-cleaving enzyme supplied a second test of the proposed chemical identity.[ref]
Isolation and partial characterization of keratan sulphate from human brain by I. Eronen and others. Abstract: isolated fraction, disaccharide backbone and enzyme susceptibility; Biochimica et Biophysica Acta 843, 155–158, 1985
The fraction also spanned a range of molecular sizes in size-exclusion chromatography. A material can therefore share a characteristic backbone while containing chains of different lengths. Combining chemical composition, size separation and a cleavage test makes the identification more informative than simply detecting a negatively charged substance in a tissue sample.[ref]
Isolation and partial characterization of keratan sulphate from human brain by I. Eronen and others. Abstract: gel-filtration heterogeneity and repeating sulfated structure
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