An intersection pairing counts signed intersections of transverse representatives of complementary homology classes, using one relative representative when the manifold has boundary. With coefficients it is defined without orientation choices. Poincare-Lefschetz duality makes this a perfect pairing for compact manifolds.
The intersection matrix records an intersection pairing on chosen classes. Its nonsingularity proves linear independence: a relation between the classes, paired with each class in turn, gives a vector in the matrix's kernel.
In the product , for , vary the factors indexed by an -element subset and fix points in the other factors. Orient the resulting submanifold in increasing factor order. These homology classes form an integral middle-dimensional basis by the Künneth theorem. Complementary subsets give a transverse intersection at one point, with positive sign since all factor dimensions are even. For noncomplementary subsets some factor is fixed in both representatives; moving that point makes them disjoint. In particular every basis class has zero self-intersection number. Complementary pairs give hyperbolic blocks in the intersection form.
For a compact three-manifold , let . The boundary intersection pairing satisfies : the long exact sequence in relative homology identifies with boundaries of relative surfaces, and the boundary-interior adjoint identity identifies its orthogonal complement with the same kernel. Hence . This also implies that a closed surface with odd first mod-two Betti number, such as , cannot be the entire boundary of a compact three-manifold.

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