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Joined 2026-09-21 Message
Incoming links: Le Cong
Genome editing 2026-11-05
Genome editing deliberately changes the DNA sequence at selected sites in a genome. Changes can include a small insertion or deletion, replacement of a sequence, or introduction of a specific base change. This differs from merely measuring gene expression or transiently changing it without altering the underlying DNA.
In a common CRISPR-Cas9 approach, a guide RNA directs a nuclease to a matching DNA region, and cellular DNA repair determines the resulting sequence change. Repair can disrupt a gene or use a supplied template to introduce a designed change. Le Cong and colleagues demonstrated programmable targeting of endogenous mammalian sites and simultaneous editing at several sites in 2013.[ref]
Multiplex genome engineering using CRISPR/Cas systems by Le Cong and others. Abstract and Results: endogenous loci, repair pathways and multiplex targeting
A targeted cut is therefore an intermediate operation, not proof of the intended edit. DNA sequencing of the resulting alleles distinguishes the planned change from unintended repair products.
Multiplex genome engineering using CRISPR/Cas systems by Le Cong and others Created 2026-10-20 Updated 2026-10-31