Masasuke Yoshida coauthored direct observation of rotation in isolated F1-ATPase. A fluorescent filament attached to the rotary system made angular motion visible during ATP-driven activity.
This converts an inferred coupling mechanism into a mechanical readout. The isolated motor assay establishes rotation; demonstrating membrane-coupled ATP synthesis requires a different experimental configuration.[ref]
Figure 1.
Portrait of Masasuke Yoshida
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