Past exam of the biochemistry and Molecular Biology course of the University of Cambridge / 2025 / Paper 2 / Section C / 14 / a

One candidate inward-facing PCR primer pair, written $5^{\prime }$ to $3^{\prime }$, isThe forward PCR primer binds the intronic flank immediately upstream of exon 5; the reverse PCR primer is the reverse complement of a downstream exon-5 segment. Their amplicon includes the repeat while both binding sites lie outside it. Using the displayed reference sequence gives an approximately $122$-base-pair product; an indel changes the intervening length and sequence. The primers have matched simple Wallace-rule estimates near $60{}^{\circ }\mathrm{C}$. These are candidates from the supplied sequence: confirm genomic specificity, absence of variable primer-binding sites, secondary structures and actual reaction conditions before use. Sequence from a unique flank toward the repeat and verify a single specific product.