The liposomes could be separated from free proteins by density-gradient flotation: lipid-containing particles float to a different position from soluble protein. Size-exclusion chromatography is another possibility if it resolves the large vesicles from the unincorporated proteins. Simple sedimentation can also collect sufficiently large vesicles, but protein aggregates may sediment too. The important control is to show that the recovered SNARE proteins associate with the lipid bilayer, rather than merely copurifying with it.

Ancestors (13)

  1. 1
  2. Section 1
  3. Paper 5
  4. 2025
  5. Past exam of the biochemistry course of the University of Cambridge
  6. Biochemistry course of the University of Cambridge
  7. Year 3 of the natural sciences course of the University of Cambridge
  8. Year of the natural sciences course of the University of Cambridge
  9. Natural sciences course of the University of Cambridge
  10. Course of the University of Cambridge
  11. University of Cambridge
  12. List of universities
  13. Home

View article source

Discussion (0)

New discussion

There are no discussions about this article yet.

Articles by others on the same topic (0)

There are currently no matching articles.
See all articles in the same topic Create my own version