After warming, more labelled v-SNARE remains full length: lane 2 is approximately 80–85% of the reference signal, compared with about 40–45% in lane 1. Formation of a protected SNARE complex provides a consistent explanation: the susceptible part of v-SNARE is less accessible after complex assembly.
Protection is not itself a unique readout of membrane fusion. Some protein may face the vesicle lumen, and SNARE complexes can protect protein without proving that the two aqueous compartments have merged. The inference depends on comparing these alternatives with the detergent and partner-free controls.

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