For a nonnegative random variable and , Markov inequality states
For a random variable of finite mean and variance , Chebyshev inequality states that for every ,
To prove it, apply Markov inequality to the nonnegative variable at threshold :
For an indicator , . Covariances vanish for independent pairs. For dependent distinct pairs,
Expanding the variance, with ordered pairs as in the definition of , gives
For sufficiently large , . By Chebyshev inequality,
This is the second moment method: a vanishing relative variance makes a zero count unlikely.
A threshold function has the following meaning: if , the probability of a tends to zero; if , that probability tends to one. Behavior at a fixed constant multiple is not specified by this definition.
Let count the five-vertex subsets spanning in the Erdős-Rényi model . Then
If , then , so Markov inequality gives .
Now let with . Then . Distinct copies have dependent indicators only if their vertex sets share at least two vertices. If they share vertices, they share edges; there are ordered such pairs, and their joint probability is . Consequently
Part (b) gives . Therefore
This is the five-vertex instance of the fixed-size clique appearance threshold.

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