The Snake lemma applies to a commutative diagram of modules with exact rows and , and vertical maps . It supplies the exact sequence
For , choose with . Commutativity gives , so for a unique . Define modulo . Changing by changes by , proving well-definedness. This construction is additive. Exactness follows by the same lift-and-chase: exactly when the lift can be adjusted into ; a class in maps to zero in exactly when it is obtained from such a lift. The remaining kernel and cokernel positions follow directly from row exactness.
For an open cover , use the short exact sequence of singular chain complexes
where the last complex consists of chains whose simplices lie in one member of the cover. This is exact because a simplex present in both summands lies in their intersection. Barycentric subdivision and its chain homotopy to the identity show that this small-chain inclusion induces an isomorphism on homology: compactness of each simplex and a Lebesgue-number argument place sufficiently subdivided simplices inside the cover.
Applying the Snake lemma to a short exact sequence of chain complexes gives the connecting homomorphism : lift a quotient cycle to the middle complex, take its differential, and identify that differential with an element of the subcomplex. Its independence of the lift and representative follows by the same chase. Repeating this degree by degree, or chasing the cycles and boundaries directly, gives
This is the Mayer–Vietoris sequence. Specifically, split a small cycle as ; then lies in the intersection, and with the sign convention above.
For the final truncation, use homological grading . The span in degrees below is a subcomplex. The span called in degrees at least need not be a subcomplex with the inherited differential: may lie in . Give the quotient differential, with zero and all higher differentials inherited. Then
is a short exact sequence of chain complexes. Its only potentially nonzero connecting map is
Indeed ; is a cycle in , and changing by changes by zero. In other degrees either the source or the relevant target of the connecting map vanishes.

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