Past exam of the mathematics course of the University of Cambridge / 2017 / iii / Paper 338 / 3 / c / iv / Solution

If spatial illumination is stable between the two sums, it acts just like the sensitivity factor: the mean ratio remains one but each detector pixel has a different shot-noise variance. A whole-image standard deviation is disproportionately influenced by faint detector pixels, so substituting a global arithmetic mean signal generally underestimates detector conversion gain. If the illumination pattern differs between the sums, their noise-free ratio is not constant; its structure adds apparent variance and causes a further systematic error.

Use locally uniform signal bins or subregions, remove offsets, mask defective or saturated detector pixels, and propagate the local shot and read variances. In the stable-pattern case a useful alternative to pooling raw ratios is the normalized differenceAt high signal and negligible read noise, treating the denominator at its mean gives $\mathrm{Var}(U_i)\simeq (2N_i/g)/(2N_i)=1/g$, independent of the illumination. Thus a variance fit or $g\simeq 1/\mathrm{Var}(U)$ can use a spatially varying flat without the arithmetic/harmonic mismatch. Measured noisy denominators cause higher-order corrections, so independent or smoothed signal estimates can be preferable. Fit and subtract a genuine changing illumination pattern before noise analysis, using sufficient smoothing not to fit away the random noise. Arbitrary flat-field normalization without propagating its noise does not by itself recover the original gain.