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The respiratory quotient compares carbon dioxide production with molecular oxygen consumption over the same interval. Complete glucose oxidation in the net equation above gives $\mathrm{RQ}=6/6=1$. In the ideal respirometer gas balance with no absorber, $f=0$, that conversion gives $D=0$: substantial cellular respiration can occur without any net gas contraction. A zero contraction therefore does not by itself demonstrate zero metabolism.

Other fuels, incomplete conversion and concurrent synthesis can alter the ratio. Absorber-based respirometry isolates molecular oxygen uptake by deliberately removing the other gas signal; it cannot independently determine the respiratory quotient or identify glucose as the consumed fuel. Separate carbon dioxide measurement would supply the missing account.