Sequencing of RNA fragments protected by ribosomes to measure their positions on transcripts. Occupancy measures a combination of entry, passage and dwell time; more footprints can reflect slower elongation rather than more completed protein.
Methods that preferentially capture ribosomes near biological translation starts, often using short inhibitor treatments. Candidate peaks require calibration and independent validation.
Frame-related enrichment at a three-base interval in correctly positioned translating-ribosome reads. It supports biological translation but is difficult to establish for very short or low-count reading frames.
An RNA fragment protected from digestion by a ribosome in the assayed preparation. Length, genomic position and frame periodicity help distinguish productive biological translation from other protected RNA.
Profiling of preserved ribosomal and small-subunit-associated complexes to distinguish stages including scanning and recycling. Captured distributions remain subject to crosslinking and fragment-selection biases.
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