Single-cell RNA sequencing

Measurement of RNA profiles in individual cells. It resolves heterogeneous populations, but transcript abundance alone does not prove lineage potential, signalling dependence or new transcription.

An inferred ordering of molecular states along a developmental trajectory. It does not itself establish which cell divided to produce another cell.

An inferred ordering of biological cells by a model of state progression. It is not by itself a measured time, a lineage trace or proof of causal developmental transitions.