Detection of individual RNA molecules by multiple sequence-complementary fluorescent probes. Probe specificity, molecule detection and compartment boundaries require validation before interpreting subcellular enrichment.
Identification of many nucleic acid targets through repeated rounds of fluorescent hybridization and imaging. Matching signals across rounds connects temporal coding with spatially retained molecular identities.
A highly multiplexed imaging approach extending sequential RNA hybridization through additional spatial and temporal coding. It connects repeated fluorescence measurements with transcriptome-scale identification while retaining positions in tissue.[ref]
An RNA-imaging method using combinatorial codes with error-detection or correction properties. Redundant measurements help distinguish a true molecular identity from an imperfect sequence of observed signals.[ref]
Spatially resolved, highly multiplexed RNA profiling in single cells by Kok Hao Chen and others. Encoding and error robustness
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