Stern-Volmer equation (${\Phi }_0/{\Phi }_Q=1+k_Q{\tau }_0[Q]$)

For purely dynamic quenching of a single emitting population, ${\Phi }_0/{\Phi }_Q={\tau }_0/{\tau }_Q=1+k_Q{\tau }_0[Q]$. The slope measures $k_Q{\tau }_0$, so determining a bimolecular quenching constant requires the unquenched lifetime or equivalent independent rate information.