NMR methods selecting slowly relaxing coherence components through interference between relaxation mechanisms. Appropriate labelling and field conditions extend measurements to larger assemblies.
Methyl-based NMR strategies exploiting favourable relaxation properties of selected methyl coherences, often with selective isotopic labelling in a largely deuterated protein.
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Transverse Relaxation-Optimized Spectroscopy (TROSY) is a technique used in nuclear magnetic resonance (NMR) spectroscopy that helps improve the sensitivity and resolution of signals from macromolecules, particularly large proteins and protein complexes. In NMR, the relaxation of the magnetic moments of nuclei can lead to signal loss over time, especially in large molecules where relaxation times can be significantly longer due to internal motion.