TurboID is an engineered promiscuous biotin ligase used for proximity labeling. A fusion can label nearby proteins during a short pulse, while localization-matched and no-added-biotin controls help distinguish the intended neighbourhood from background.[ref]
Efficient proximity labeling in living cells and organisms with TurboID by Tess C. Branon and others. Fig. 2, short-pulse compartment labeling and specificity comparisons
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