Xueguang Sun studied the structure and RNA-binding properties of the bacterial protein Hfq. His Georgia Tech doctoral thesis, dated May2006, identifies Roger M. Wartell as his committee chair and documents structural analysis, binding experiments and studies of RNA association.[ref]
Characterization of E coli Hfq structure and its RNA binding properties by Xueguang Sun. Title and approval pages; chaptersII–IV
The thesis combines predicted protein structure with experiments probing RNA binding, including gel electrophoresis and fluorescence anisotropy. That combination tests a structural hypothesis against binding behavior rather than treating a predicted surface as sufficient evidence of its function.[ref]
Characterization of E coli Hfq structure and its RNA binding properties by Xueguang Sun. ChapterIII, sections on gel mobility shift, fluorescence anisotropy and mutant binding
Sun later coauthored the2007 frozen-solution study with J. Michael Li and Wartell, showing that a stable RNA hairpin could convert efficiently into a metastable RNA dimer under freezing conditions.[ref]
These projects connect molecular recognition with competing structures: a protein can present alternative binding surfaces, and an RNA strand can pair internally or associate with another strand. Experiments are needed to distinguish those possibilities; a sequence or a mobility band alone does not specify the resulting complex.
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