An overview of recent non-standard Bitcoin transactions by 0xB10C Updated 2025-05-23 +Created 1970-01-01
Running:gives:
hd hello_world.out
00000000 7f 45 4c 46 02 01 01 00 00 00 00 00 00 00 00 00 |.ELF............|
00000010 02 00 3e 00 01 00 00 00 b0 00 40 00 00 00 00 00 |..>.......@.....|
00000020 40 00 00 00 00 00 00 00 10 01 00 00 00 00 00 00 |@...............|
00000030 00 00 00 00 40 00 38 00 02 00 40 00 06 00 03 00 |....@.8...@.....|
00000040 01 00 00 00 05 00 00 00 00 00 00 00 00 00 00 00 |................|
00000050 00 00 40 00 00 00 00 00 00 00 40 00 00 00 00 00 |..@.......@.....|
00000060 d7 00 00 00 00 00 00 00 d7 00 00 00 00 00 00 00 |................|
00000070 00 00 20 00 00 00 00 00 01 00 00 00 06 00 00 00 |.. .............|
00000080 d8 00 00 00 00 00 00 00 d8 00 60 00 00 00 00 00 |..........`.....|
00000090 d8 00 60 00 00 00 00 00 0d 00 00 00 00 00 00 00 |..`.............|
000000a0 0d 00 00 00 00 00 00 00 00 00 20 00 00 00 00 00 |.......... .....|
000000b0 b8 01 00 00 00 bf 01 00 00 00 48 be d8 00 60 00 |..........H...`.|
000000c0 00 00 00 00 ba 0d 00 00 00 0f 05 b8 3c 00 00 00 |............<...|
000000d0 bf 00 00 00 00 0f 05 00 48 65 6c 6c 6f 20 77 6f |........Hello wo|
000000e0 72 6c 64 21 0a 00 2e 73 79 6d 74 61 62 00 2e 73 |rld!...symtab..s|
000000f0 74 72 74 61 62 00 2e 73 68 73 74 72 74 61 62 00 |trtab..shstrtab.|
00000100 2e 74 65 78 74 00 2e 64 61 74 61 00 00 00 00 00 |.text..data.....|
00000110 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
*
00000150 1b 00 00 00 01 00 00 00 06 00 00 00 00 00 00 00 |................|
00000160 b0 00 40 00 00 00 00 00 b0 00 00 00 00 00 00 00 |..@.............|
00000170 27 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |'...............|
00000180 10 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
00000190 21 00 00 00 01 00 00 00 03 00 00 00 00 00 00 00 |!...............|
000001a0 d8 00 60 00 00 00 00 00 d8 00 00 00 00 00 00 00 |..`.............|
000001b0 0d 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
000001c0 04 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
000001d0 11 00 00 00 03 00 00 00 00 00 00 00 00 00 00 00 |................|
000001e0 00 00 00 00 00 00 00 00 e5 00 00 00 00 00 00 00 |................|
000001f0 27 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |'...............|
00000200 01 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
00000210 01 00 00 00 02 00 00 00 00 00 00 00 00 00 00 00 |................|
00000220 00 00 00 00 00 00 00 00 90 02 00 00 00 00 00 00 |................|
00000230 08 01 00 00 00 00 00 00 05 00 00 00 07 00 00 00 |................|
00000240 08 00 00 00 00 00 00 00 18 00 00 00 00 00 00 00 |................|
00000250 09 00 00 00 03 00 00 00 00 00 00 00 00 00 00 00 |................|
00000260 00 00 00 00 00 00 00 00 98 03 00 00 00 00 00 00 |................|
00000270 4c 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |L...............|
00000280 01 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
00000290 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
000002a0 00 00 00 00 00 00 00 00 00 00 00 00 03 00 01 00 |................|
000002b0 b0 00 40 00 00 00 00 00 00 00 00 00 00 00 00 00 |..@.............|
000002c0 00 00 00 00 03 00 02 00 d8 00 60 00 00 00 00 00 |..........`.....|
000002d0 00 00 00 00 00 00 00 00 01 00 00 00 04 00 f1 ff |................|
000002e0 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
000002f0 11 00 00 00 00 00 02 00 d8 00 60 00 00 00 00 00 |..........`.....|
00000300 00 00 00 00 00 00 00 00 1d 00 00 00 00 00 f1 ff |................|
00000310 0d 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 |................|
00000320 00 00 00 00 04 00 f1 ff 00 00 00 00 00 00 00 00 |................|
00000330 00 00 00 00 00 00 00 00 2d 00 00 00 10 00 01 00 |........-.......|
00000340 b0 00 40 00 00 00 00 00 00 00 00 00 00 00 00 00 |..@.............|
00000350 34 00 00 00 10 00 02 00 e5 00 60 00 00 00 00 00 |4.........`.....|
00000360 00 00 00 00 00 00 00 00 40 00 00 00 10 00 02 00 |........@.......|
00000370 e5 00 60 00 00 00 00 00 00 00 00 00 00 00 00 00 |..`.............|
00000380 47 00 00 00 10 00 02 00 e8 00 60 00 00 00 00 00 |G.........`.....|
00000390 00 00 00 00 00 00 00 00 00 68 65 6c 6c 6f 5f 77 |.........hello_w|
000003a0 6f 72 6c 64 2e 61 73 6d 00 68 65 6c 6c 6f 5f 77 |orld.asm.hello_w|
000003b0 6f 72 6c 64 00 68 65 6c 6c 6f 5f 77 6f 72 6c 64 |orld.hello_world|
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000003d0 73 5f 73 74 61 72 74 00 5f 65 64 61 74 61 00 5f |s_start._edata._|
000003e0 65 6e 64 00 |end.|
000003e4
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it PCR verification with gel electrophoresis Updated 2025-05-23 +Created 1970-01-01
For this reason, it is wise to verify that certain steps are correct whenever possible.
Gel electrophoresis separates molecules by their charge-to-mass ratio. It is one of those ultra common lab procedures!
Since we know that we amplified the 16S regions which we know the rough size of (there might be a bit of variability across species, but not that much), we were expecting to see a big band at that size.
And that is exactly what we saw!
First we had to prepare the gel, put the gel comb, and pipette the samples into wells present in the gel:
To see the DNA, we added ethidium bromide to the samples, which is a substance that that both binds to DNA and is fluorescent.
Because it interacts heavily with DNA, ethidium bromide is a mutagen, and the biology people sure did treat the dedicated electrophoresis bench area with respect! Figure 4. "Gel electrophoresis dedicated bench area to prevent ethidium bromide contamination.".
Gel electrophoresis dedicated bench area to prevent ethidium bromide contamination.
Source. Gel electrophoresis dedicated waste bin for centrifuge tubes and pipette tips contaminated with ethidium bromide.
Source. The UV transilluminator we used to shoot UV light into the gel was the Fisher Scientific UVP LM-26E Benchtop 2UV Transilluminator. The fluorescent substance then emitted a light we can see.
As barely seen at Figure 8. "Fischer Scientific UVP LM-26E Benchtop 2UV Transilluminator illuminated gel." due to bad photo quality due to lack of light, there is one strong green line, which compared to the ladder matches our expected 16S length. What we saw it with the naked eyes was very clear however.
By the creator of SymPy, who works at Los Alamos National Laboratory and has a PhD in chemical physics: swww.linkedin.com/in/ondřej-čertík-064b355b/ Man, big kudos to this dude.
Sometimes systems of Diophantine equations are considered.
Problems generally involve finding integer solutions to the equations, notably determining if any solution exists, and if infinitely solutions exist.
The general problem is known to be undecidable: Hilbert's tenth problem.
The Pythagorean triples, and its generalization Fermat's last theorem, are the quintessential examples.
Spin like mad between:
- standards
- high level generators. We use the assembler
as
and linkerld
. - hexdumps
- file decompilers. We use
readelf
. It makes it faster to read the ELF file by turning it into human readable output. But you must have seen one byte-by-byte example first, and think howreadelf
output maps to the standard. - low-level generators: stand-alone libraries that let you control every field of the ELF files you generated. github.com/BR903/ELFkickers, github.com/sqall01/ZwoELF and many more on GitHub.
- consumer: the
exec
system call of the Linux kernel can parse ELF files to starts processes: github.com/torvalds/linux/blob/v4.11/fs/binfmt_elf.c, stackoverflow.com/questions/8352535/how-does-kernel-get-an-executable-binary-file-running-under-linux/31394861#31394861
- Compiler toolchains generate and read ELF files.
- Operating systems read and run ELF files.
- Specialized libraries. Examples:
This is a term "invented" by Ciro Santilli to refer to quantum compilers that are able to convert non-specifically-quantum (functional, since there is no state in quantum software) programs into quantum circuit.
The term is made by adding "quantum" to the more "classical" concept of "high-level synthesis", which refers to software that converts an imperative program into register transfer level hardware, typicially for FPGA applications.
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