Ciro Santilli @cirosantilli 35

They are not public in any meaningful modern sense, just like "public universities" in the United Kingdom: are public universities in the UK owned by the Government?.

With all this ready, we opened the Nanopore flow cell, which is the 500 dollar consumable piece that goes in the sequencer.

We then had to pipette the final golden Eppendorf into the flow cell. My anxiety levels were going through the roof: Figure 4. "Oxford nanopore MinION flow cell pipette loading.".

At this point bio people start telling lab horror stories of expensive solutions being spilled and people having to recover them from fridge walls, or of how people threw away golden Eppendorfs and had to pick them out of trash bins with hundreds of others looking exactly the same etc. (but also how some discoveries were made like this). This reminded Ciro of: youtu.be/89UNPdNtOoE?t=919 Alfred Maddock's plutonium spill horror story.

Luckily this time, it worked out!

We then just had to connect the MinION to the computer, and wait for 2 days.

During this time, the DNA would be sucked through the pores.

As can be seen from Video 1. "Oxford Nanopore MinION software channels pannel on Mac." the software tells us which pores are still working.

Pores go bad sooner or later randomly, until there are none left, at which point we can stop the process and throw the flow cell away.

48 hours was expected to be a reasonable time until all pores went bad, and so we called it a day, and waited for an email from the PuntSeq team telling us how things went.

We reached a yield of 16 billion base pairs out of the 30Gbp nominal maximum, which the bio people said was not bad.

The first/only way Ciro could find was with ampy: stackoverflow.com/questions/74150782/how-to-run-a-micropython-host-script-file-on-the-raspbery-pi-pico-from-the-host/74150783#74150783 That just worked and it worked perfectly!

TODO: possible with rshell?

Examples at: Raspberry Pi Pico W MicroPython example.

This section is about partial implementations that are only able to read the blocks, ususally coming from Bitcoin Core, to interpret the data:

Space between the inner and bacterial outer membrane in Gram-negative bacteria

Completeness: math.stackexchange.com/questions/2192665/is-this-set-of-bessel-functions-a-basis-for-all-c10-a-functions TODO

This is the Bessel function analogue to Fourier basis is complete for $L^2$.

This should only be used for things that happen outside of the state that React trackes, e.g. window event handlers.

Examples:

No support:Example with raw examples under nodejs/sequelize/raw/many_to_many.js

The most common way to construct multi-qubit gates is to use single-qubit gates as part of a controlled quantum gate.

Bibliography:

The Hadamard gate takes $|0⟩$ or $|1⟩$ (quantum states with probability 1.0 of measuring either 0 or 1), and produces states that have equal probability of 0 or 1.

www-thphys.physics.ox.ac.uk/people/AndreiStarinets/sr_mt_2022.html (archive) contains 2022 problem sets and notes, well done Mr Andrei Starinets!

Europe has made good regulations to limit the absolute power of immoral companies. Partly because it does not have any companies anymore, but so be it.

But the law that forces every fucking website to show a message "Do you consent to cookies?" is not one of them.

Ciro cannot stand fucking clicking the "I consent" button anymore.

Please stop, for the love of God.

At most, there must be a standardized API that allows your browser to say "I agree or I disagree" automatically to all of them, e.g. an HTTP header.

2021: United Kingdom is considering it post-Brexit: techcrunch.com/2021/09/06/after-years-of-inaction-against-adtech-uks-ico-calls-for-browser-level-controls-to-fix-cookie-fatigue/. Something good might actually come out of Brexit!

It hasn't achieved anything beyond the Schengen zone.

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