Ciro Santilli @cirosantilli 35

Also known as: Quantitative PCR (qPCR).

Like PCR, but the amplification machine measures the concentration of DNA at each step.

This describes one possible concentration detection method with fluorescent molecules that only become fluorescent when the DNA is double stranded (SYBR Green)

This allows you to predict the exact initial concentration by extrapolating the exponential curve backwards.

TODO: vs non-real-time PCR. Why can't you just divide by 2 for every heating step to reach back the original concentration? Likely the reaction reach saturation at an unknown step.

TODO: vs non-real-time PCR in medical diagnostics: do you really need to know concentration for diagnostics? Isn't it enough to know if the virus is present or not?

This game (1 or 2, can't remember) made an impression on Ciro Santilli for some reason.

Only many many years after playing it, after Ciro started getting more interested, did he learn that it was actually an adaptation of the Chinese mega-classic Water Margin.

"Suikoden" is the actual Japenese transliteration for the Chinese name of the original Water Margin novel.

Yet another case of Chinese thing better known in the West as Japanese.

The game puts great emphasis on the concept of the 108 Stars of Destiny, which never left Ciro's mind: making 108 allies, the main collectible of the game, allows you to make a more powerful alliance, and unlock better endings.

This seems to be the "brown Brazilian bean" that many Brazilians eat every day.

Edit: after buying it, not 100% sure. This one felt smaller than what Ciro had in Brazil, borlotti beans might be closer. Pinto beans are smaller, and creamier, and have softer peel, possibly produced less natural gas.

2021-04: second try.

2021-03: did for first time, started with same procedure as borlotti beans 2021-03. Maybe 1h30 is too much. Outcome was still very good.

From Wikipedia:

Vaporware?

Good modern OpenGL tutorial in retained mode with shaders, see also: stackoverflow.com/questions/6733934/what-does-immediate-mode-mean-in-opengl/36166310#36166310

A problem such that all NP problems can be reduced in polynomial time to it.