An amplicon is a piece of DNA that has been amplified, typically through a process like polymerase chain reaction (PCR). This amplification process allows for the creation of millions of copies of a specific DNA sequence, which can then be used for various purposes in molecular biology, genetics, and biotechnology. Amplicons are often used in various applications including: 1. **Genetic Testing**: To identify genetic mutations or variations associated with diseases.
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An amplicon is a nucleic acid product of amplification. In ordinary PCR amplification without added primer tails, its boundaries eventually correspond to the outer ends of the two PCR primer binding sites. First-cycle extensions on long starting templates can run beyond the opposite site; exact-length single strands appear in cycle 2 and exact-length duplex products in cycle 3 under complete extension. This explains why the bounded product becomes dominant even though the initial extensions need not have its length.