Molecular weight cut-off (MWCO) refers to the maximum molecular weight of a substance that can pass through a semipermeable membrane or filter. It is a critical parameter used in various applications, including protein purification, dialysis, ultrafiltration, and other processes in biochemistry and molecular biology. MWCO is typically expressed in daltons (Da) or kilodaltons (kDa) and indicates the size or molecular weight at which molecules are retained by the membrane.
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