For B-splines and interpolation sites , the B-spline collocation matrix is . Its invertibility is the existence-and-uniqueness condition for interpolation in that B-spline basis.
For increasing sites and an ordered nonnegative normalized B-spline basis, the matrix is a totally nonnegative matrix. One way to see the minors' signs is knot insertion. Each inserted knot changes coefficients by with , using the standard constant endpoint pieces. This is a nonnegative rectangular bidiagonal map, whose minors are nonnegative. Products preserve that property by the Cauchy–Binet formula. Insert each site to full knot multiplicity; a suitable refined coefficient is then the value . Thus collocation is an ordered row submatrix of the refinement map, and has nonnegative minors. The strict support conditions give invertibility by the Schoenberg–Whitney theorem; its inverse has the checkerboard inverse of a totally nonnegative matrix sign pattern.
For increasing interpolation sites and an order- B-spline basis with knots , the B-spline collocation matrix is invertible exactly when
for every , equivalently when for every .

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