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Measured half-integer spin and fermionic behavior rule out a simple quark-antiquark pair: two spin-$1/2$ constituents have integer total spin, and adding integer orbital angular momentum does not change that. Their measured baryon number $B=1$, inferred through production and conservation bookkeeping, corresponds to $n_q-n_{\bar{q}}=3$. Their pattern of electric charges, strangeness and isospin then agrees with three light valence quarks.

Baryon magnetic moments provide a more detailed check. The additive quark model gives, for example,Approximately equal up and down constituent masses give ${\mu }_u\simeq -2{\mu }_d$, hence ${\mu }_p/{\mu }_n\simeq -3/2$, close to the measured ratio of about $-1.46$.

These observations support three valence quarks; they do not prove the absence of extra quark-antiquark pairs. For instance, $qqqq\bar{q}$ also has $B=1$ and can have half-integer spin. Ordinary Quantum chromodynamics baryons also contain sea pairs and gluons. The stronger interpretation that the measurements exclude every antiquark component is therefore false.