Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery by Mei-Chu Lo and others (2004)

By Mei-Chu Lo and colleagues. Analytical Biochemistry332(1),153–159(1September2004). DOI doi.org/10.1016/j.ab.2004.04.031

Abstract: environmentally sensitive dye readout, ligand/control thermal shift, commercial temperature/fluorescence instrument, comparison with isothermal titration calorimetry and activity assays.