actin patches initially return in a depolarized distribution and then become concentrated at a growth pole. The depolarized fraction peaks near 20 minutes and subsequently falls as the polarized fraction rises. Patch assembly therefore recovers before normal spatial organization is restored.
A plausible cellular interpretation is recovery from disrupted trafficking and growth polarity, followed by establishment of a bud-associated growth site and renewed polarized secretion and endocytosis. Continued cell-cycle progression can also redistribute actin patches as biological cells grow and prepare to divide. The graph alone does not show that all biological cells pass synchronously through one particular cell-cycle stage, or that the recovered pole is the same pole used before treatment. Time-lapse imaging of the same biological cells and a bud or cell-cycle marker would distinguish restoration of an old site from selection of a new one.
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