For the real coefficients of the stated self-adjoint Sturm-Liouville problem, define the weighted inner product
Since , a nonzero eigenfunction has positive squared norm. Let . Integration by parts and the Dirichlet boundary conditions give
Taking to be an eigenfunction shows that its eigenvalue is real, since is real. For two eigenfunctions with eigenvalues , the same identity gives
Hence distinct eigenvalues imply orthogonality in the weighted inner product. This is the orthogonality of Sturm-Liouville eigenfunctions. The calculation assumes the regularity needed for the stated self-adjoint boundary problem, so all boundary terms and integrals are defined.

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