At 298 K with a=0.5μm, this gives approximately 4.37×10−13m2s−1. The argument assumes an overdamped particle in an equilibrium thermal bath, rather than arbitrary driven diffusion.
The sketch has two wells separated by 1 micrometre, with the secondfree-energy level higher by kBTlog2 and an intervening escape barrier. Free energy includes the volume of each trapped basin; equal minimum potential heights alone would not ensure equal occupations if basin shapes differed.