Slide-seq

Slide-seq measures gene expression with spatial information by transferring tissue-associated RNA onto a surface carrying position-mapped barcoded beads. Sequencing associates captured transcripts with bead barcodes and hence with locations. The original study used approximately ten-micrometre beads and demonstrated tissue expression patterns at high spatial resolution. A bead measurement is a capture region rather than an automatic identification of one intact cell; tissue geometry and capture efficiency still affect interpretation. ^[ref]