Ciro Santilli @cirosantilli 35

Ciro Santilli's eulogy: effortless effort

Quantum field theory lecture by Tobias Osborne (2017) mentions that quantization is a guess.

For Fractional quantum Hall effect for $\nu =1/m$.

When the process changes, cr3 change to point to the page table of the new current process.

This creates a problem: the TLB is now filled with a bunch of cached entries for the old process.

A simple and naive solution would be to completely invalidate the TLB whenever the cr3 changes.

However, this is would not be very efficient, because it often happens that we switch back to process 1 before process 2 has completely used up the entire TLB cache entries.

The solution for this is to use so called "Address Space Identifiers" (ASID) as mentioned in sources such as:

Basically, the OS assigns a different ASID for each process, and then TLB entries are automatically also tagged with that ASID. This way when the process makes an access, the TLB can determine if a hit is actually for the current process, or if it is an old address coincidence with another process.

The x86 also offers the invlpg instruction which explicitly invalidates a single TLB entry. Other architectures offer even more instructions to invalidated TLB entries, such as invalidating all entries on a given range.

For the discovery of green fluorescent protein.

One of the most beautiful molecular machines known!

The first one with such complexity that was uncovered.

The thing rotates like a water wheel for God's sake, except it uses protons instead of water.

The ATP synthase complex is so large that Power, Sex, Suicide by Nick Lane (2006) page 123 contains a cryoEM image of several ATP synthases on small membrane vesicles, this is the paper: pubs.acs.org/doi/abs/10.1021/bi00437a031# under a fucking paywall.

Ciro Santilli's sport of choice circa 2020, see also: Ciro Santilli's cycling.

What do you prefer, 1 \times 10^{10} or 1E10.

www.threekit.com/blog/gltf-everything-you-need-to-know comparision of several formats

One cool thing we did in this procedure was to use magnetic separation with magnetic beads to further concentrate the DNA: Figure 1. "GE MagRack 6 pipetting.".

The beads are coated to stick to the DNA, which allows us to easily extract the DNA from the rest of the solution. This is cool, but bio people are borderline obsessed by those beads! Go figure!

Then we prepared the DNA for sequencing with the Oxford Nanopore specific part: Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit.

Here some of the steps required a bit more of vortexing for mixing the reagents, and for this we used the VELP Scientifica WIZARD IR Infrared Vortex Mixer which appears to be quicker to use and not as strong as the Vortex Genie 2 previously used to break up the cells:

After all that was done, the DNA of the several 400 ml water bottles and hours of hard purification labour were contained in one single Eppendorf!