You can connect form an Ubuntu 22.04 host as:When in but be aware of: Raspberry Pi Pico W freezes a few seconds after after screen disconnects from UART.
screen /dev/ttyACM0 115200screen, you can Ctrl + C to kill main.py, and then execution stops and you are left in a Python shell. From there:- Ctrl + D: reboots
- Ctrl + A K: kills the GNU screen window. Execution continues normally
Other options:
- ampy
runcommand, which solves How to run a MicroPython script from a file on the Raspberry Pi Pico W from the command line?
Superconducting qubits are good because superconductivity is macroscopic by 
Ciro Santilli 35 Updated 2025-04-18 +Created 1970-01-01
Ciro Santilli 35 Updated 2025-04-18 +Created 1970-01-01Superconducting qubits are regarded as promising because superconductivity is a macroscopic quantum phenomena of Bose Einstein condensation, and so as a macroscopic phenomena, it is easier to control and observe.
This is mentioned e.g. in this relatively early: physicsworld.com/a/superconducting-quantum-bits/. While most quantum phenomena is observed at the atomic scale, superconducting qubits are micrometer scale, which is huge!
Physicists are comfortable with the use of quantum mechanics to describe atomic and subatomic particles. However, in recent years we have discovered that micron-sized objects that have been produced using standard semiconductor-fabrication techniques – objects that are small on everyday scales but large compared with atoms – can also behave as quantum particles.
PuntSeq is a side project led by a few University of Cambridge PhDs that aims to determine which bacteria are present in the River Cam.
In July 2019, the PuntSeq team got together with the awesome Cambridge Biomakespace, an awesome biology makerspace open to all, to create a two day science outreach activity showing their procedures.
The data collected in this experiment, together with other collection sessions done by the organizers actually led to a publication on eLife: elifesciences.org/articles/61504 "Freshwater monitoring by nanopore sequencing" by Lara Urban et al. (2021), so it is awesome to see that were are actual being part of "real science".
Ciro knows nothing about biology, but since he is very curious about it, he jumped at this opportunity, and decided to document things as well as his limited knowledge would allow.
All participants chipped in some money to help cover the experiment's costs. Ciro suspects that this activity was done partially to help crowdfund the experiment, but it was a worthy investment!
The impressions you get from the experiment as a software engineer will be:
- OMG, this is so labour intensive, why haven't they automated this
- OMG, this is frightening, all the 8 hours of work I've just done are present in that tiny plastic tube
- Amazing! Look at that apparatus! And the bio people are like: I've used this a million times, it's cheap and every lab has one, just work faster and don't break you piece of junk!
More generic PCR information at: Section "Polymerase chain reaction".
Because it is considered the less interesting step, and because it takes quite some time, this step was done by the event organizers between the two event days, so participants did not get to take many photos.
PCR protocols are very standard it seems, all that biologists need to know to reproduce is the time and temperature of each step.
This process used a Marshal Scientific MJ Research PTC-200 Thermal Cycler:
We added PCR primers for regions that surround the 16S DNA. The primers are just bought from a vendor, and we used well known regions are called 27F and 1492R. Here is a paper that analyzes other choices: academic.oup.com/femsle/article/221/2/299/630719 (archive) "Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment" by Yuichi Hongoh, Hiroe Yuzawa, Moriya Ohkuma, Toshiaki Kudo (2003)
One cool thing about the PCR is that we can also add a known barcode at the end of each primer as shown at Code 1. "PCR diagram".
This means that we bought a few different versions of our 27F/1492R primers, each with a different small DNA tag attached directly to them in addition to the matching sequence.
This way, we were able to:
- use a different barcode for samples collected from different locations. This means we
- did PCR separately for each one of them
- for each PCR run, used a different set of primers, each with a different tag
- the primer is still able to attach, and then the tag just gets amplified with the rest of everything!
- sequence them all in one go
- then just from the sequencing output the barcode to determine where each sequence came from!
Input: Bacterial DNA (a little bit)
... --- 27S --- 16S --- 1492R --- ...
|||
|||
vvv
Output: PCR output (a lot of)
Barcode --- 27S --- 16S --- 1492RCode 1.
PCR diagram
. Finally, after purification, we used the Qiagen QIAquick PCR Purification Kit protocol to purify the generated from unwanted PCR byproducts.
Nick Leeson and the Fall of the House of Barings by Adam Curtis (1996) by Ciro Santilli 35 Updated 2025-04-18 +Created 1970-01-01
Ciro Santilli 35 Updated 2025-04-18 +Created 1970-01-01 lujakob/nestjs-realworld-example-app SQLite port by Ciro Santilli 35 Updated 2025-04-18 +Created 1970-01-01
Ciro Santilli 35 Updated 2025-04-18 +Created 1970-01-01Tried a quick port to SQLite to get rid of annoying local databases for development, but failed, at c1c2cc4e448b279ff083272df1ac50d20c3304faandthen:fails with:Attempt to hack it:and after that it seems to run.
npm install sqlite3 --save-dev{
"type": "sqlite",
"database": "db.sqlite3",
"entities": ["src/**/**.entity{.ts,.js}"],
"synchronize": true
}npm startDataTypeNotSupportedError: Data type "timestamp" in "ArticleEntity.created" is not supported by "sqlite" database.--- a/src/article/article.entity.ts
+++ b/src/article/article.entity.ts
@@ -20,10 +20,10 @@ export class ArticleEntity {
@Column({default: ''})
body: string;
- @Column({ type: 'timestamp', default: () => "CURRENT_TIMESTAMP"})
+ @Column({ default: () => "CURRENT_TIMESTAMP"})
created: Date;
- @Column({ type: 'timestamp', default: () => "CURRENT_TIMESTAMP"})
+ @Column({ default: () => "CURRENT_TIMESTAMP"})
updated: Date;I can signup and login, terrible error reporting as usual, make sure to use long enough usernames/passwords.
However, article creation fails with:
Unhandled Rejection (TypeError): Cannot read property 'slug' of undefinedhello_world_len points to the special st_shndx == SHN_ABS == 0xF1FF.0xF1FF is chosen so as to not conflict with other sections.st_value == 0xD == 13 which is the value we have stored there on the assembly: the length of the string Hello World!.This is small optimization that our assembler does for us and which has ELF support.
The "last" gene, and also an E. Coli K-12 MG1655 gene of unknown function.
A collection of closely related and curated C. elegans datasets.
It does not appear possible to achieve the other two levels besides SERIALIZABLE and READ UNCOMMITED
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