archean/proterozoic barrier.
Program Raspberry Pi Pico W with MicroPython code from the command line by 
Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16 How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit by Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16Repairs the ends of DNA, and also attaches an adapter protein to the DNA that makes them go through the pores of e.g. an Oxford Nanopore MinION.
How to use an Oxford Nanopore MinION to extract DNA from river water and determine which bacteria live in it Filtration with vacuum pump by Ciro Santilli 37 Updated 2025-07-16
Ciro Santilli 37 Updated 2025-07-16The first thing we did was to filter the water samples with a membrane filter that is so fine that not even bacteria can pass through, but water can.
Therefore, after filtration, we would have all particles such as bacteria and larger dirt pieces in the filter.
From the 1 liter in each bottle, we only used 400 ml because previous experiments showed that filtering the remaining 600 ml is very time consuming because the membrane filter gets clogged up.
Therefore, the filtration step allows us to reduce those 400 ml volumes to more manageable Eppendorf tube volumes: Figure 1. "An Eppendorf tube". Reagents are expensive, and lab bench centrifuges are small!
Labelled Eppendorf tubes on a rack
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Since the filter is so fine, filtering by gravity alone would take forever, and so we used a vacuum pump to speed thing up!
For that we used:
Peeling the vacuum pump filter protection peel before usage
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Placing the vacuum pump filter
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arstechnica.com/information-technology/2016/11/private-microwave-networks-financial-hft/ The secret world of microwave networks (2016) Fantastic article.
Example:
- the three most table polymorphs of calcium carbonate polymorphs are:
Last updated 2018 as of 2021, but still just worked.
Gotta run github.com/feathersjs/feathers-chat first: github.com/feathersjs-ecosystem/feathers-chat-react/issues/5, then it worked:and on the other terminal:then visit localhost:3000/ and you can create an account and login, tested on Ubuntu 20.10. Data is stored on persistently.
git clone https://github.com/feathersjs/feathers-chat
cd feathers-chat
git checkout fd729a47c57f9e6170cc1fa23cee0c84a004feb5
npm install
npm startgit clone https://github.com/feathersjs-ecosystem/feathers-chat-react
cd feathers-chat-react
git checkout 36d56cbe80bbd5596f6a108b1de9db343b33dac3
npm install
npm startIf you disable JavaScript on Chromium, it stops working completely. There is a section on how to solve that at: docs.feathersjs.com/cookbook/express/view-engine.html but it does not cover React specifically. Codaisseur/feathersjs-react-redux-ssr might be good to look into.
Allow us to determine with good approximation in a multi-electron atom which electron configuration have more energy. It is a bit like the Aufbau principle, but at a finer resolution.
Note that this is not trivial since there is no explicit solution to the Schrödinger equation for multi-electron atoms like there is for hydrogen.
For example, consider carbon which has electron configuration 1s2 2s2 2p2.
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