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Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay by Regina Bruggisser and others by
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Primary abstract and byline: Regina Bruggisser, Katrin von Daeniken, Gernot Jundt, Willi Schaffner and Heide Tullberg-Reinert. Planta Medica 68(5), 445–448 (May 2002), DOI 10.1055/s-2002-32073. Cell number assessed by crystal violet declined while MTT formazan increased under kaempferol treatment. Cell-free tests showed direct reduction with kaempferol, resveratrol and selected extracts. These observations motivate chemical and optical controls; they do not make every positive assay signal a cell count.
Additional verified locator: final two sentences of the primary abstract, PMID 12058323. Washing kaempferol-treated MCF7 and HS578 cells before adding MTT significantly reduced direct dye reduction. The comparison supports chemical interference under those assay conditions; it does not show that washing eliminates all interference or reverses prior biological exposure. pubmed.ncbi.nlm.nih.gov/12058323/?dopt=Abstract
Primary source: www.urmc.rochester.edu/labs/mosmann
Verified locator: Official laboratory homepage names Tim R. Mosmann and directly lists the 1983 assay with PMID 6606682. Lab Members www.urmc.rochester.edu/labs/mosmann/lab-members names Tim R. Mosmann, describes cytokine/T-cell research and explicitly identifies the Tim Mosmann portrait link www.urmc.rochester.edu/neuroscience/_shared/img/faculty/TimMosmann.jpg . No expansion of R. or birth date.
Primary source: www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/191/466/11465007001.pdf
Verified locator: Manufacturer instructions, version 20, content version December 2020. Printed p. 3 names the MTT bromide reagent; §3.1 and Fig. 4 on printed p. 9 show yellow tetrazolium reduction to purple formazan, solubilization and multiwell optical measurement. Publisher-hosted document retains Roche kit branding.
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays by T. Mosmann by
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T. Mosmann, Journal of Immunological Methods 65(1–2), 55–63 (16 December 1983), DOI 10.1016/0022-1759(83)90303-4. Primary Abstract: a tetrazolium-based colorimetric method detects living mammalian cells, with signal depending on cellular activation, and is read using a multiwell scanning spectrophotometer. Exact initial-only byline retained.
Verified author linkage: the official Rochester Mosmann laboratory homepage www.urmc.rochester.edu/labs/mosmann names Tim R. Mosmann and directly lists this 1983 paper with PMID 6606682 in Selected Publications. The Lab Members page independently names Tim R. Mosmann. Preserve the paper’s literal T. Mosmann byline and original source title; the canonical person/Work now use Tim R. Mosmann, with both old IDs retained as aliases. R. remains unexpanded.
Biomechanical ordering of dense cell populations by Dmitri Volfson and others by
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CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein by S. D. Wright and others by
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Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus by M. Desjardins and others by
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Journal of Cell Biology124(1994),677–688; DOI10.1083/jcb.124.5.677. Primary abstract and Figures5–7: J-774 macrophage latex-bead phagosomes isolated on sucrose gradients, pulse/chase analysis and changes in membrane markers. Initial-only author form retained; full name not inferred from secondary indexes.
Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule by M. D. Pierschbacher and E. Ruoslahti by
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Identification of the membrane glycoprotein that is the C3b receptor of the human erythrocyte, polymorphonuclear leukocyte, B lymphocyte, and monocyte by D. T. Fearon by
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Journal of Experimental Medicine152(1),20–30(1980); abstract: erythrocyte membrane gp205, C3-convertase decay and C3b-inactivator-assisted cleavage; receptor identification across blood-cell classes.
Identification and characterization of a novel monocyte subpopulation in human peripheral blood by B. Passlick and others by
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Blood74(7),2527–2534(1989). Abstract: two-color immunofluorescence/flow cytometry, CD14/CD16 coexpression, approximately13percent of CD14-defined monocytes in reported samples; morphology and functional comparisons.
Phagocytosing macrophages exclude proteins from the zones of contact with opsonized targets by S. D. Wright and S. C. Silverstein by
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Original abstract: protein access beneath macrophages on glass versus ligand-coated glass; access by molecular mass200000 in the former condition and exclusion even at50000 in the latter. Byline remains initial-only; a functional contact seal is supported without asserting that every molecule is impermeable.
Receptors for C3b and C3bi promote phagocytosis but not the release of toxic oxygen from human phagocytes by S. D. Wright and S. C. Silverstein by
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By S. D. Wright and S. C. Silverstein. Journal of Experimental Medicine158(6),2016–2023(1December1983). DOI doi.org/10.1084/jem.158.6.2016
Abstract and Figure1: ligand-coated surfaces, cultured monocytes and fibronectin; complement-receptor-associated phagocytosis can be separated from hydrogen-peroxide release. C3bi is the historical spelling used by the paper.
ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18) by Michael S. Diamond and others by
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Journal of Cell Biology111(1990),3129–3139; DOI10.1083/jcb.111.6.3129. Original full byline verifies Michael S.Diamond. Abstract and reciprocal-binding assays: purified proteins, engineered cells and antibody blocking establish Mac-1/ICAM-1 interaction; stimulated neutrophil/endothelial conjugates examined. Slash in source title must become hyphen in magic-link argument.
Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1) by Steven D. Marlin and Timothy A. Springer by
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Cell51(1987),813–819; DOI10.1016/0092-8674(87)90104-8. Full primary publisher byline verifies Steven D.Marlin and Timothy A.Springer. Abstract: purified ICAM-1 in supported lipid membranes; LFA-1-positive/negative cell comparison and antibody blocking. Existing T.A.Springer author/Work by records refer to this collaborator; do not create a duplicate full-name article.
Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor by D. I. Beller and others by
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T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1 by M. L. Dustin and T. A. Springer by
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Adenosine triphosphate-induced sliding of tubules in trypsin-treated flagella of sea-urchin sperm by K. E. Summers and I. R. Gibbons by
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Identification of distinct luminal domains for macromolecules, erythrocytes, and leukocytes within mammalian capillaries by H. Vink and B. R. Duling by
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Circulation Research79(3),581–589 (1996), DOI10.1161/01.res.79.3.581. Primary indexed abstract: bright-field and fluorescence microscopy of hamster cremaster capillaries, distinct tracer/blood-cell domains and light-dye perturbation. Direct PubMed page returned an incomplete response; indexed primary abstract inspected. Initials retained as published.
Asymmetric budding of viruses in epithelial monlayers: a model system for study of epithelial polarity by E. Rodriguez Boulan and D. D. Sabatini by
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PNAS 75(10), 5071–5075 (1978), DOI 10.1073/pnas.75.10.5071. Indexed title spelling monlayers retained. Primary archive pmc.ncbi.nlm.nih.gov/articles/PMC336265/ ; author-institution record vivo.weill.cornell.edu/display/pubid283416 . Abstract and electron-microscopic observations: polarized budding in epithelial monolayers.
Pinned article: Introduction to the OurBigBook Project
Welcome to the OurBigBook Project! Our goal is to create the perfect publishing platform for STEM subjects, and get university-level students to write the best free STEM tutorials ever.
Everyone is welcome to create an account and play with the site: ourbigbook.com/go/register. We belive that students themselves can write amazing tutorials, but teachers are welcome too. You can write about anything you want, it doesn't have to be STEM or even educational. Silly test content is very welcome and you won't be penalized in any way. Just keep it legal!
Intro to OurBigBook
. Source. We have two killer features:
- topics: topics group articles by different users with the same title, e.g. here is the topic for the "Fundamental Theorem of Calculus" ourbigbook.com/go/topic/fundamental-theorem-of-calculusArticles of different users are sorted by upvote within each article page. This feature is a bit like:
- a Wikipedia where each user can have their own version of each article
- a Q&A website like Stack Overflow, where multiple people can give their views on a given topic, and the best ones are sorted by upvote. Except you don't need to wait for someone to ask first, and any topic goes, no matter how narrow or broad
This feature makes it possible for readers to find better explanations of any topic created by other writers. And it allows writers to create an explanation in a place that readers might actually find it.Figure 1. Screenshot of the "Derivative" topic page. View it live at: ourbigbook.com/go/topic/derivativeVideo 2. OurBigBook Web topics demo. Source. - local editing: you can store all your personal knowledge base content locally in a plaintext markup format that can be edited locally and published either:This way you can be sure that even if OurBigBook.com were to go down one day (which we have no plans to do as it is quite cheap to host!), your content will still be perfectly readable as a static site.
- to OurBigBook.com to get awesome multi-user features like topics and likes
- as HTML files to a static website, which you can host yourself for free on many external providers like GitHub Pages, and remain in full control
Figure 2. You can publish local OurBigBook lightweight markup files to either OurBigBook.com or as a static website.Figure 3. Visual Studio Code extension installation.Figure 5. . You can also edit articles on the Web editor without installing anything locally. Video 3. Edit locally and publish demo. Source. This shows editing OurBigBook Markup and publishing it using the Visual Studio Code extension. - Infinitely deep tables of contents:
All our software is open source and hosted at: github.com/ourbigbook/ourbigbook
Further documentation can be found at: docs.ourbigbook.com
Feel free to reach our to us for any help or suggestions: docs.ourbigbook.com/#contact





