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A merging galaxy cluster exhibiting the bullet Cluster dark-matter separation: peaks of weak gravitational lensing mass lie nearer the galaxies than the displaced X-ray gas. Combining the components supports a dominant less-collisional gravitating component under the usual gravitational model.^[ref]

The inference of dark matter uses a gravitational law to turn observed motions and lensing into a mass distribution. modified gravity asks whether some discrepancies instead reveal a breakdown of that law. The strongest motivation at galactic scales is the regular relation between baryonic structure and galaxy rotation curves, including approximately flat outer rotation speeds and the baryonic Tully-Fisher relation.

In modified Newtonian dynamics, the low-acceleration spherical relation is $g\mu (g/a_0)=g_N$, with $\mu (x)\to 1$ at high acceleration and $\mu (x)\to x$ at low acceleration. For an isolated baryonic mass $M_b$ in the latter regime,Thus an asymptotically flat rotation curve and a fourth-power mass–speed relation arise from a common acceleration scale, without assigning an independent halo profile to every galaxy. This is a substantive phenomenological motivation. Distances, stellar mass-to-light ratios, gas distributions and the external gravitational environment must still be controlled.^[ref]

A nonrelativistic acceleration prescription does not itself specify light bending or cosmology. A relativistic completion must determine the metric followed by photons and matter. tensor-vector-scalar gravity illustrates the need for additional dynamical fields and a specified physical metric; lensing predictions then have to be calculated within that theory rather than imported unchanged from general relativity.^[ref]

Several observations challenge a simple replacement of dark matter by a single galactic acceleration law. galaxy clusters retain substantial mass discrepancies even after such a modification. In the bullet Cluster dark-matter separation, the dominant X-ray-emitting baryonic gas is displaced from the galaxies, whereas weak-lensing mass concentrations lie nearer the galaxies. Collisionless dark matter accompanying the galaxies provides a natural account of this separation. The inference uses a lensing model and does not constitute a theorem excluding every conceivable modified-gravity theory, but a successful alternative must reproduce the displaced lensing signal quantitatively.^[ref]

The cosmic microwave background, acoustic structure, expansion history and growth of cosmic structure provide additional simultaneous tests. A theory that fits galaxy rotation curves must also supply the appropriate cosmological perturbation evolution and lensing. Adding extra clustering fields can help, but may reintroduce an effective unseen gravitating component. Solar-system and binary-pulsar agreement with general relativity constrain alternatives in their tested regimes, although a modification designed to act only at very low acceleration is not automatically ruled out by a high-acceleration test.

A discriminating programme compares dynamics, which probes the potential accelerating nonrelativistic matter, with lensing, which probes the sum of the two scalar metric potentials. Scale-dependent discrepancies, gravitational slip and environmental screening can then be tested with controlled galaxy bias and selection effects. Galaxy-scale tensions in a dark-matter model must likewise be separated from uncertain baryonic astrophysics. Regular galactic scaling relations motivate modified gravity; clusters and the combined cosmological evidence strongly challenge its simplest dark-matter-free forms. Failure to detect a particular WIMP is not positive evidence for a particular modified-gravity theory.

gravitational lensing measures the gravitational influence of matter without requiring that matter to shine. In general relativity a point mass produces small-angle deflectionFor a thin lens, with angular-diameter distances $D_d,D_s,D_{ds}$,Image multiplicity, distortion and magnification therefore constrain projected gravitating mass, with source and lens geometry measured separately.

Strong lensing gives multiple images, arcs and Einstein rings. Their positions and, where available, time delays constrain a central mass distribution. Weak lensing measures correlated small image distortions over a larger field. Using the critical surface density for gravitational lensing and reduced gravitational shear, the convergence and shear satisfyBecause intrinsic galaxy shapes are unknown, weak shear is inferred statistically using many sources. Point-spread-function anisotropy, shear calibration, photometric redshifts, intrinsic alignments and foreground contamination must be controlled.

An inferred lensing mass can be compared with the independently estimated stellar and hot-gas mass. The bullet Cluster dark-matter separation provides a particularly informative separation of components: the collisional X-ray-emitting gas is displaced from the galaxies, while lensing mass peaks approximately follow the less-collisional galaxy component. The primary analysis combined wide-field and Hubble imaging rather than inferring lensing mass from X-ray hydrostatic equilibrium.^[ref] This favors a dominant relatively collisionless unseen component. Translating a merger offset into a particle self-interaction cross section also requires a merger model and projection uncertainties; lensing does not directly reveal a WIMP's mass or quantum numbers.

Microlensing tests a different possibility: dark matter in compact objects. A compact foreground lens can create an unresolved, time-dependent magnification of a background star. Event frequency and duration constrain the abundance and mass range of compact lenses only after survey cadence, detection efficiency, blending, source sizes and halo geometry are included. Achromaticity and the expected light-curve shape help distinguish simple lensing from intrinsic variability. A null survey result does not exclude every compact-object mass at every location.

Weak-lensing correlations and small-scale strong-lens structure also test the matter power spectrum and halo substructure, potentially distinguishing cold from warm free-streaming behavior. Baryonic feedback can change the same small-scale mass distribution. There are intrinsic inversion ambiguities: the mass-sheet transformationleaves reduced gravitational shear unchanged. Source-redshift information, absolute magnification or other data can restrict such degeneracies. Lensing establishes where gravitating matter is and tests its distribution and interactions; identification of its particle species requires complementary evidence.