A DNA ladder contains DNA fragments of known lengths in base pairs. Its gel bands supply size references. Match the sample's strandedness and shape, and use the specified lengths for that DNA ladder. A size reference need not provide a quantitative mass standard.[ref]
Five Main Nucleic Acid Gel Electrophoresis Steps by Thermo Fisher Scientific. Step 2, Nucleic acid ladder selection guidelines: fragment structure, conformation, intended use and manufacturer-specific composition
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