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Joined 2026-09-21 Message
Incoming links: Maurice B. Burg
Carl W. Gottschalk by Maurice B. Burg 2026-11-05
By Maurice B. Burg.
Verified locator: Biographical Memoirs77(1999), chapter Carl W. Gottschalk, opening p123: dates28April1922–15October1997 and byline MauriceB.Burg. Early research/micropuncture sections pp126–130: collaboration with Margaret Mylle, local fluid sampling, osmolality, dye/microdissection site verification. DOI10.17226/9681. Colleague-authored institutional memoir; original experimental observations separately cited to1959 paper.
Mark A. Knepper 2026-11-05
Mark A. Knepper is a researcher in kidney physiology who uses systems biology to investigate the regulation of water transport. A central example is how vasopressin changes the distribution of aquaporin-2 between the plasma membrane and the interior of collecting duct cells. His 2010 research report describes combining mass spectrometry, measurements of gene expression, mathematical modeling and physiological experiments to investigate this regulation.[ref]
Solute And Water Transport In Renal Epithelia by Mark A. Knepper. NIH RePORTER project 1ZIAHL001285-24, fiscal year 2010; Abstract
The timing gives the mechanism its interest. On a scale of seconds to minutes, membrane trafficking can move existing aquaporin-2 into the plasma membrane, changing the route available for water movement. That is a different regulatory step from making additional aquaporin-2 through altered gene expression. Measuring how much channel protein exists and where it is located therefore answers different questions.[ref]
Solute And Water Transport In Renal Epithelia by Mark A. Knepper. Abstract: rapid redistribution between membrane and cytoplasm
Mark A. Knepper also wrote a scientific remembrance of Maurice B. Burg, explaining how experiments on isolated renal tubules opened individual transport mechanisms to direct investigation.[ref]
Maurice B. Burg (1931–2022), discoverer of kidney transport mechanisms by Mark A. Knepper. Opening and discussion of the isolated-perfused-tubule method; PNAS 119, e2209749119, 2022
Maurice B. Burg 2026-11-05
Maurice B. Burg was a researcher in renal physiology who developed experimental methods for studying individual nephron segments outside the intact kidney. Isolated tubule perfusion made it possible to relate transport properties to a particular anatomical segment, rather than observing only the final composition of urine.[ref]
Maurice B. Burg (1931–2022), discoverer of kidney transport mechanisms by Mark A. Knepper. Opening remembrance: invention and use of isolated-perfused-nephron techniques
The 1966 method paper by Maurice B. Burg and collaborators described preparations from single rabbit nephrons. Supplying fluid through an isolated renal tubule while controlling its surrounding solution allows the luminal and external conditions to be varied separately.[ref]
Preparation and study of fragments of single rabbit nephrons by M. Burg and others. Original1966 method-paper record and preparation
A useful experiment is to change the surrounding osmolality and compare entering and collected tubular fluid. A change in collected volume can reveal net water movement; changes in solute content address transport of dissolved substances. Isolating the segment constrains where the measured exchange occurred. The preparation simplifies the system while retaining an organized living epithelium, an important middle scale between a whole organ and an isolated protein.