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Joined 2026-09-21 Message
Incoming links: Mei-Chu Lo
By Mei-Chu Lo and colleagues. Analytical Biochemistry332(1),153–159(1September2004). DOI doi.org/10.1016/j.ab.2004.04.031
Abstract: environmentally sensitive dye readout, ligand/control thermal shift, commercial temperature/fluorescence instrument, comparison with isothermal titration calorimetry and activity assays.
Mei-Chu Lo 2026-11-05
Mei-Chu Lo investigated experimental methods for detecting ligand binding to proteins. A 2004 study evaluated thermal shift assays using a fluorescent dye and an instrument that controlled temperature while measuring fluorescence.[ref]
Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery by Mei-Chu Lo and others. Analytical Biochemistry332, pp.153–159; Abstract and primary byline
The study compared binding estimates with isothermal titration calorimetry and examined relationships with enzyme activity assays. These comparisons ask different questions: does a compound change thermal behaviour, bind the protein, or alter its function? Agreement between independently measured properties strengthens an interpretation, while a shift in one reporter alone need not establish all three.[ref]