Motion can weigh a system whose mass is otherwise inaccessible. For a self-gravitating isolated system in virial equilibrium, let and denote equilibrium averages and use its gravitational radius and a mass-weighted mean squared speed relative to its centre of mass. Then gives . If random velocities are isotropic and mean streaming has been removed, , where is the one-dimensional line-of-sight velocity dispersion. Observations sample many objects at one epoch; replacing a time average by that sample requires a representative, approximately stationary population, beyond the trajectory identity itself.[ref]
A practical measurement route uses an optical telescope to locate members and a spectrograph to measure Doppler shifts of identified spectral lines. Their shifts relative to the system's mean give radial velocities; angular separations plus a distance estimate constrain its size. Subtracting instrumental broadening and measurement variance, testing membership, and looking for coherent rotation or subgroups help control the inference. The projected luminous size need not equal , and luminous tracers need not have the same distribution as all gravitating mass. Unknown velocity-anisotropy parameters, interlopers, mergers, external tidal forces and appreciable boundary stresses can bias a naive estimate. A large velocity dispersion alone does not establish virial equilibrium.

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